Regenerative Effect of Bone Marrow-derived Mesenchymal Stem Cells in Thioacetamide-induced Liver Fibrosis of Rats

AUTHORS

D. Mehrabani 1 , 2 , Z. Khajehahmadi 3 , * , P. Tajik 4 , A. Tamadon 5 , F. Rahmanifar 6 , M. Ashraf 7 , N. Tanideh 5 , 8 , S. Zare 5

1 Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran| Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

2 Comparative and Experimental Medicine Center, Shiraz University of Medical Sciences, Shiraz, Iran

3 Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

4 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

5 Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

6 Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

7 Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

8 Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

How to Cite: Mehrabani D, Khajehahmadi Z, Tajik P, Tamadon A, Rahmanifar F, et al. Regenerative Effect of Bone Marrow-derived Mesenchymal Stem Cells in Thioacetamide-induced Liver Fibrosis of Rats, Arch Razi Inst. 2019 ; 74(3):e98578. doi: 10.22092/ari.2018.110029.1120.

ARTICLE INFORMATION

Archives of Razi Institute: 74 (3); 279-286
Published Online: October 01, 2019
Article Type: Journal Article
Received: March 24, 2017
Accepted: September 25, 2018
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Abstract

The present study determined the regenerative effect of bone marrow-derived stem cells (BMSCs) on thioacetamide (TA)-induced liver fibrosis in rats. A total of 30 male Wistar rats were randomly divided into sham control and treatment groups. The rats of the sham control group were subdivided into three groups and sampled on the 14th, 18th, and 20th weeks after fibrosis induction. The rats of the treatment group were subdivided into two groups and sampled on the 4th and 6th weeks after BMSCs treatment. Fibrosis was induced by the intraperitoneal administration of 200 mg/kg of TA twice a week for a period of 14 weeks. All the animals underwent liver function tests and histopathologic evaluation 4 and 6 weeks after BMSCs transplantation. The BMSCs were characterized using osteogenic induction and reverse transcription-polymerase chain reaction. The BMSCs were plastic adherent, spindle-shaped, and positive for osteogenic differentiation. They expressed CD73 and were negative for CD45. The infiltration of inflammatory cells and deposition of collagen fibers were noticed after TA administration. A significant decline in inflammatory cells and a healing process were detected 4 weeks after cell transplantation. The amelioration in hepatic tissue was significant 6 weeks after cell therapy. Following the injection of BMSCs, a nonsignificant decrease was visible in aspartate transaminase level; however, this decline was significant for alanine aminotransferase level. The alkaline phosphatase and albumin levels showed an increasing trend after cell administration. The transplantation of BMSCs resulted in a significant regenerative effect after hepatic injuries. Therefore, it was shown that BMSCs transplantation can open a new window and be a therapy of choice in the amelioration of liver fibrosis.

Keywords

Bone marrow Mesenchymal stem cell Liver Thioacetamide Fibrosis Rat

© 2019, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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