Histopathology and Histomorphological Study of Wound Healing Using Clove Extract Nanofibers (Eugenol) Compared to Zinc Oxide Nanofibers on the Skin of Rats

AUTHORS

M. A. Ashjazadeh 1 , A. Jahandideh 2 , * , G. Abedi 1 , A. Akbarzadeh 3 , S. Hesaraki 4

1 Depatment of veterinary surgery, Science and research branch, Islamic Azad University, Tehran, Iran

2 Department of Clinical Science, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran| Universal Scientific Education and Research Network, Tabriz, Iran

4 Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

How to Cite: Ashjazadeh M A, Jahandideh A, Abedi G, Akbarzadeh A, Hesaraki S. Histopathology and Histomorphological Study of Wound Healing Using Clove Extract Nanofibers (Eugenol) Compared to Zinc Oxide Nanofibers on the Skin of Rats, Arch Razi Inst. 2019 ; 74(3):e98576. doi: 10.22092/ari.2018.120170.1184.

ARTICLE INFORMATION

Archives of Razi Institute: 74 (3); 267-277
Published Online: October 01, 2019
Article Type: Journal Article
Received: January 16, 2018
Accepted: April 29, 2018
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Abstract

In order to evaluate the healing effect of eugenol and other nanofibers, 100 male Wistar rats (200±10 g) were used with 14-15 weeks of age in this study. All of the male rats were transferred in the standard cages under controlled exposure conditions in a 12:12 h light/dark cycle with a constant temperature about 22±2 oC. In addition, the male rats were fed with pellets. Firstly, anesthesia process was performed by 2% xylazine hydrochloride (10mg/Kg/IP) and 10% ketamine hydrochloride (100mg/Kg/IP), and then the rats were placed on the operating table. Then the dorsal surfaces of the rats’ skin to ileum were scrubbed and prepared as the next step. A circular wound (with a 7 mm diameter) was created by a 7 mm sterile biopsy punch. All 100 rats were divided into four groups (n=25) randomly named as control, nano zinc oxide (ZnO), eugenol nanofibers, and polycaprolactone groups. After that, they were divided into five groups regarding the wound closure rate in days 3, 5, 7, 14, and 21. Then, the wound dressings were placed on the wounds and renewed every 24 h. At the end of days 3, 5, 7, 14, and 21, the relevant tests, such as histopathology, were conducted by removing the tissue volume using a biopsy punch, and then decapitation process was performed on the rats. It was obvious that eugenol nanofiber showed the best granulation tissue by the production of collagen. Further studies are being performed on wound healing by eugenol nanofiber. 

Keywords

Nanofibres Eugenol Zinc Oxide Skin Rat Wound Healing

© 2019, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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