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Evaluation of Cytotoxic Effect and Antioxidant Activity of Grape Seed Extract, Crocin, and Phenytoin

AUTHORS

N. Razmaraii 1 , 2 , H. Babaei 1 , 3 , A. Mohajjel Nayebi 3 , S. Ahdi Khosroshahi 4 , S. Farajnia 1 , *

AUTHORS INFORMATION

1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Cellular & Molecular Biotechnology, Razi Vaccine and Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

3 Department of Pathobiology, Faculty of Veterinary, University of Tehran, Tehran, Iran

4 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

ARTICLE INFORMATION

Archives of Razi Institute: 72 (3); 181-187
Published Online: September 22, 2016
Article Type: Journal Article
Received: August 18, 2016
Accepted: September 22, 2016
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Abstract

Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and phenytoin (PHEN) on a human breast cancer cell line (MCF-7). Methanol extracts of the three mentioned agents were prepared and their antioxidant activity was evaluated by diphenyl-1-picrylhydrazyl method, using Quercetine (QUER) as positive control. The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxic effect of the extracts on Michigan Cancer Foundation-7MCF-7 cell line, using doxorubicin hydrochloride (DOX) as the positive control. Given the results, greater scavenging activity was achieved by using GSE in comparison to CRO and PHEN. Further, a significant correlation was found between the antioxidant activity and cytotoxic effects of these agents, and GSE had the highest antioxidant capacity and cytotoxic effect in comparison to CRO and PHEN.

Keywords

Antioxidant Activity Crocin Cytotoxicity Grape Seed Extract

© 2017, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.
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