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The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain

AUTHORS

S. Zandieh 1 , M. Lotfi 2 , * , M. Kamalzadeh 2 , N. Shiri 2 , E. Parmour 2 , A. Eshghi 2 , S. Masoudi 3 , M. H. Hablolvarid 4 , A. Shoushtari 5 , H. Goudarzi 6 , S. M. J. Taher Mofrad 7 , S. Amanpour 7

AUTHORS INFORMATION

1 Department of Genetics, Faculty of Sciences, Islamic Azad University, Ahar, Iran

2 Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

3 Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran

4 Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

5 Management of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran

6 Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran

7 Center of Cancer Biology Research, Tehran University of Medical Sciences, Tehran, Iran

ARTICLE INFORMATION

Archives of Razi Institute: 72 (3); 173-179
Published Online: October 09, 2016
Article Type: Journal Article
Received: August 08, 2015
Accepted: October 09, 2016
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Abstract

Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.

Keywords

Infectious bursal disease Gumboro Disease Chicken embryo fibroblast Ovine lymphoid origin cell line

© 2017, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.
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