Scolicidal activity of Mesobuthus eupeus venom against the protoscolices of Echinococcus granulosus

AUTHORS

H. Jafari 1 , * , M. Nemati 1 , P. Haddad Molayan 2 , L. Khaleghi Rostamkolaie 2 , H. Hamidi Nejat 2

1 Department of Venomous Animals and Toxins. Razi Vaccine and Serum Research Institute, Ahvaz, Agriculture Research, Education and Extension Organization, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

How to Cite: Jafari H, Nemati M, Haddad Molayan P, Khaleghi Rostamkolaie L, Hamidi Nejat H. Scolicidal activity of Mesobuthus eupeus venom against the protoscolices of Echinococcus granulosus, Arch Razi Inst. 2019 ; 74(2):e95665. doi: 10.22092/ari.2018.121416.1213.

ARTICLE INFORMATION

Archives of Razi Institute: 74 (2); 183-189
Published Online: June 01, 2019
Article Type: Case Reports
Received: April 17, 2018
Accepted: September 13, 2018
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Abstract

Hydatidosis is an important zoonosis caused by a parasitic tapeworm, namely Echinococcus granulosus. This infection is distributed worldwide and affects the health as well as economic loss in both humans and animals. In most cases, the disease needs chemotherapy with or without surgery. Conventional drugs have some major problems, including drug complications, harmful side effects, and also progressive resistance. According to the importance of biological productions as alternative medicine, a large number of studies confirmed that whole venom and many peptide ingredients of the scorpion venom have various different medical benefits, including antimicrobial properties, due to the mechanism of blocking gated ion channel. In this study, the venom peptides of Mesobuthus eupeus scorpionwere purified using gel filtration chromatography and subsequently ion exchange chromatography, followed by the determination of the molecular weights of the proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) procedure. After collecting the hydatid cysts fluids from the liver of infected sheep, protoscolices were derived, washed, and encountered to the whole venom as well as eight different fractions of toxin 30, 60, 120, and 240 min after the exposure. In the next step, the viability of protoscolices was determined by eosin staining. The obtained results revealed that a venom fraction under 10 kDa killed all protoscolices after 30 min. Moreover, it was found that the scolicidal activity of fractions increases according to the time of exposure. As a result, it can be concluded that M. epeus venom peptides under its LD50 (1/2 LD50) can properly and quickly destroy the protoscolices of hydatid cysts at the level of applied concentrations and such components are good alternatives to treat hydatidosis.

© 2019, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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