Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS)

AUTHORS

M. Hematyar 1 , A. Es-haghi 2 , * , M. Soleimani 1

1 Department of Chemistry, Imam Khomeini International University (IKIU), Qazvin, Iran

2 Department of Physico Chemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

How to Cite: Hematyar M, Es-haghi A, Soleimani M. Quantification of Melittin in Iranian Honey Bee (Apis mellifera meda) Venom by Liquid Chromatography-electrospray Ionization-ion Trap Tandem Mass Spectrometry (LC-ESI-IT-MS/MS), Arch Razi Inst. 2019 ; 74(4):e100984. doi: 10.22092/ari.2018.122150.1219.

ARTICLE INFORMATION

Archives of Razi Institute: 74 (4); 435-439
Published Online: December 01, 2019
Article Type: Case Report
Received: January 12, 2020
Accepted: August 04, 2018
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Abstract

The current research aimed to quantify melittin (MEL) in Iranian honey bee (Apis mellifera meda) venom. To this end, a liquid chromatography-electrospray ionization-ion trap tandem mass spectrometry (LC-ESI-IT-MS/MS) approach was employed. Melittin is the main toxic peptide of honey bee venom with various biological and pharmacological activities. It was extracted with pure water from the bee venom samples. The analyses were performed on XBridge BEH300 C4 column using a gradient method with the mobile phase consisting of ultrapure water and acetonitrile (containing 0.1% formic acid). Signals of the melittin were recorded with the selected reaction monitoring (SRM) mode, which is a quantitative approach capable of quantifying analyte peptides with high sensitivity and. The mass spectrum of MEL was obtained in the positive ion mode and the quantification analysis was performed using precursor to product ion transition of m/z 570.2/669.9. This method demonstrated good linearity (R2˃0.997) in the range of 1-100 µg mL-1, with a limit of quantification (LOQ) of 1.0 µg mL-1. The content of MEL in Iranian honey bee venom accounts for 43–55% of total dry weight. This method can be used to evaluate the quality and authenticity of bee venom samples for different therapeutic applications of MEL.

© 2019, Archives of Razi Institute. Razi Vaccine and Serum Research Institute.

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